Structure and functional impact of seed region variant in MIR-499 gene family in bronchial asthma.

ABSTRACT:

Background

Small non-coding RNAs (microRNAs) have been evolved to master numerous cellular processes. Genetic variants within microRNA seed region might influence microRNA biogenesis and function. The study aimed at determining the role of microRNA-499 (MIR-499) gene family polymorphism as a marker for susceptibility and progression of bronchial asthma and to analyze the structural and functional impact of rs3746444 within the seed region.

Methods

Genotyping for 192 participants (96 patients and 96 controls) in the discovery phase and 319 subjects (115 patients and 204 controls) in the replication phase was performed via Real Time-Polymerase Chain Reaction technology. Patients underwent the methacholine challenge test and biochemical analysis. Gene structural and functional

SUBMITTER: Toraih EA 

PROVIDER: S-EPMC5591547 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature