Particle bombardment and subcellular protein localization analysis in the aquatic plant Egeria densa.

ABSTRACT: Particle bombardment is a powerful and relatively easy method for transient expression of genes of interest in plant cells, especially those that are recalcitrant to other transformation methods. This method has facilitated numerous analyses of subcellular localization of fluorescent fusion protein constructs. Particle bombardment delivers genes to the first layer of plant tissue. In leaves of higher plants, epidermal cells are the first cell layer. Many studies have used the epidermal cell layer of onion bulb (Allium cepa) as the experimental tissue, because these cells are relatively large. However, onion epidermal cells lack developed plastids (i.e., chloroplasts), thereby precluding subcellular localization analysis of chloroplastic proteins. In this study, we developed a protoc

SUBMITTER: Osaki Y 

PROVIDER: S-EPMC5592081 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature