Improving Acetic Acid Production by Over-Expressing PQQ-ADH in Acetobacter pasteurianus.

ABSTRACT: Pyrroquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) is a key enzyme in the ethanol oxidase respiratory chain of acetic acid bacteria (AAB). To investigate the effect of PQQ-ADH on acetic acid production by Acetobacter pasteurianus JST-S, subunits I (adhA) and II (adhB) of PQQ-ADH were over-expressed, the fermentation parameters and the metabolic flux analysis were compared in the engineered strain and the original one. The acetic acid production was improved by the engineered strain (61.42 g L^-1) while the residual ethanol content (4.18 g L^-1) was decreased. Analysis of 2D maps indicated that 19 proteins were differently expressed between the two strains; of these, 17 were identified and analyzed by mass spectrometry and two-dimensional gel electro

SUBMITTER: Wu X 

PROVIDER: S-EPMC5592214 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature