Arabidopsis calmodulin-like protein CML36 is a calcium (Ca²⁺) sensor that interacts with the plasma membrane Ca²⁺-ATPase isoform ACA8 and stimulates its activity.

ABSTRACT: Calmodulin-like (CML) proteins are major EF-hand-containing, calcium (Ca²⁺)-binding proteins with crucial roles in plant development and in coordinating plant stress tolerance. Given their abundance in plants, the properties of Ca²⁺ sensors and identification of novel target proteins of CMLs deserve special attention. To this end, we recombinantly produced and biochemically characterized CML36 from Arabidopsis thaliana We analyzed Ca²⁺ and Mg²⁺ binding to the individual EF-hands, observed metal-induced conformational changes, and identified a physiologically relevant target. CML36 possesses two high-affinity Ca²⁺/Mg²⁺ mixed binding sites and two low-affinity Ca²⁺-specific sites. Binding of Ca²⁺ i

SUBMITTER: Astegno A 

PROVIDER: S-EPMC5592680 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature