Murine chronic lymph node window for longitudinal intravital lymph node imaging.

ABSTRACT: Chronic imaging windows in mice have been developed to allow intravital microscopy of many different organs and have proven to be of paramount importance in advancing our knowledge of normal and disease processes. A model system that allows long-term intravital imaging of lymph nodes would facilitate the study of cell behavior in lymph nodes during the generation of immune responses in a variety of disease settings and during the formation of metastatic lesions in cancer-bearing mice. We describe a chronic lymph node window (CLNW) surgical preparation that allows intravital imaging of the inguinal lymph node in mice. The CLNW is custom-made from titanium and incorporates a standard coverslip. It allows stable longitudinal imaging without the need for serial surgeries while preserving lymph

SUBMITTER: Meijer EFJ 

PROVIDER: S-EPMC5592697 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature