Identification of key genes associated with the effect of osmotic stimuli on intervertebral discs using microarray analysis.

ABSTRACT: The present study aimed to explore the effect of osmotic stimuli on intervertebral discs (IVDs) using microarray analysis. Gene expression dataset GSE1648 was downloaded from the Gene Expression Omnibus database. There were 11 IVD cell samples in this dataset, which included 4 hyperosmotic stimuli samples, 3 hypoosmotic stimuli samples and 4 isosmotic stimuli samples. The differentially expressed genes (DEGs) in hyperosmotic or hypoosmotic IVD cells (designated DEGs-hyper or DEGs-hypo) were identified, compared with isosmotic cells, using the limma package of R software. The Database for Annotation, Visualization and Integrated Discovery was used to perform a Gene Ontology (GO) term enrichment analysis for the DEG sets. Protein-protein interaction (PPI) network and microRNA (miRNA) gene-re

SUBMITTER: Ni G 

PROVIDER: S-EPMC5592857 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature