Genome-Wide Screen for Genes Involved in Caenorhabditis elegans Developmentally Timed Sleep.

ABSTRACT: In Caenorhabditis elegans, Notch signaling regulates developmentally timed sleep during the transition from L4 larval stage to adulthood (L4/A) . To identify core sleep pathways and to find genes acting downstream of Notch signaling, we undertook the first genome-wide, classical genetic screen focused on C. elegans developmentally timed sleep. To increase screen efficiency, we first looked for mutations that suppressed inappropriate anachronistic sleep in adult hsp::osm-11 animals overexpressing the Notch coligand OSM-11 after heat shock. We retained suppressor lines that also had defects in L4/A developmentally timed sleep, without heat shock overexpression of the Notch coligand. Sixteen suppressor lines with defects in developmentally timed sleep were identifi

SUBMITTER: Huang H 

PROVIDER: S-EPMC5592919 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature