Ontology highlight
ABSTRACT:
SUBMITTER: Taniuchi M
PROVIDER: S-EPMC5592968 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Diagnostic microbiology and infectious disease 20120425 2
Detection of diarrheagenic Escherichia coli (DEC) typically depends on identification of virulence genes from stool cultures, not on stool itself. We developed a multiplex polymerase chain reaction (PCR) assay that detects key DEC virulence genes (stx1, stx2, eae, bfpA, ipaH, LT, STh, aaiC, aatA). The assay involved a multiplex PCR reaction followed by detection of amplicon(s) using Luminex beads. The assay was evaluated on over 100 colony and broth specimens. We then evaluated the assay using D ...[more]