Optogenetic approach for targeted activation of global calcium transients in differentiated C2C12 myotubes.

ABSTRACT: Excitation-contraction coupling in muscle cells is initiated by a restricted membrane depolarization delimited within the neuromuscular junction. This targeted depolarization triggers an action potential that propagates and induces a global cellular calcium response and a consequent contraction. To date, numerous studies have investigated this excitation-calcium response coupling by using different techniques to depolarize muscle cells. However, none of these techniques mimic the temporal and spatial resolution of membrane depolarization observed in the neuromuscular junction. By using optogenetics in C2C12 muscle cells, we developed a technique to study the calcium response following membrane depolarization induced by photostimulations of membrane surface similar or narrower than the neur

SUBMITTER: Sebille S 

PROVIDER: S-EPMC5593883 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature