Elongation factor Tu is a multifunctional and processed moonlighting protein.

ABSTRACT: Many bacterial moonlighting proteins were originally described in medically, agriculturally, and commercially important members of the low G + C Firmicutes. We show Elongation factor Tu (Ef-Tu) moonlights on the surface of the human pathogens Staphylococcus aureus (Sa_Ef-Tu) and Mycoplasma pneumoniae (Mpn_Ef-Tu), and the porcine pathogen Mycoplasma hyopneumoniae (Mhp_Ef-Tu). Ef-Tu is also a target of multiple processing events on the cell surface and these were characterised using an N-terminomics pipeline. Recombinant Mpn_Ef-Tu bound strongly to a diverse range of host molecules, and when bound to plasminogen, was able to convert plasminogen to plasmin in the presence of plasminogen activators. Fragments of Ef-Tu retain binding capabilities to host

SUBMITTER: Widjaja M 

PROVIDER: S-EPMC5593925 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature