Molecular details of secretory phospholipase A₂ from flax (Linum usitatissimum L.) provide insight into its structure and function.

ABSTRACT: Secretory phospholipase A₂ (sPLA₂) are low molecular weight proteins (12-18 kDa) involved in a suite of plant cellular processes imparting growth and development. With myriad roles in physiological and biochemical processes in plants, detailed analysis of sPLA₂ in flax/linseed is meagre. The present work, first in flax, embodies cloning, expression, purification and molecular characterisation of two distinct sPLA₂s (I and II) from flax. PLA₂ activity of the cloned sPLA₂s were biochemically assayed authenticating them as bona fide phospholipase A₂. Physiochemical properties of both the sPLA₂s revealed they are thermostable proteins requiring di-valent cations for optimum activity.While, structural analys

SUBMITTER: Gupta P 

PROVIDER: S-EPMC5593939 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature