Efficient identification of SNPs in pooled DNA samples using a dual mononucleotide addition-based sequencing method.

ABSTRACT: Identifying single nucleotide polymorphism (SNPs) from pooled samples is critical for many studies and applications. SNPs determined by next-generation sequencing results may suffer from errors in both base calling and read mapping. Taking advantage of dual mononucleotide addition-based pyrosequencing, we present Epds, a method to efficiently identify SNPs from pooled DNA samples. On the basis of only five patterns of non-synchronistic extensions between the wild and mutant sequences using dual mononucleotide addition-based pyrosequencing, we employed an enumerative algorithm to infer the mutant locus and estimate the proportion of mutant sequence. According to the profiles resulting from three runs with distinct dual mononucleotide additions, Epds could recover the mutant bases. Results s

SUBMITTER: Cao C 

PROVIDER: S-EPMC5594057 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature