The mouse cortical meninges are the site of immune responses to many different pathogens, and are accessible to intravital imaging.

ABSTRACT: A wide range of viral and microbial infections are known to cause meningitis, and there is evidence that the meninges are the gateway to pathogenic invasion of the brain parenchyma. Hence observation of these regions has wide application to understanding host-pathogen interactions. Interactions between pathogens and cells of the immune response can be modified by changes in their environment, such as suppression of the flow of blood and lymph, and, particularly in the case of the meninges, with their unsupported membranes, invasive dissection can alter the tissue architecture. For these reasons, intravital imaging through the unperforated skull is the method of choice. We give a protocol for a simple method of two-photon microscopy through the thinned cortical skull of the anesthetized mou

SUBMITTER: Coles JA 

PROVIDER: S-EPMC5595162 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature