Role of Microbiota in Strengthening Ocular Mucosal Barrier Function Through Secretory IgA.

ABSTRACT:

Purpose

The purpose of this study was to evaluate mechanisms controlling secretory IgA (SIgA) production, thereby ensuring maintenance of ocular surface health.

Methods

To determine whether the presence of specific gut commensal species regulates SIgA levels and IgA transcripts in the eye-associated lymphoid tissues (EALT), specific-pathogen-free (SPF) Swiss Webster (SW) mice were treated with antibiotic cocktails, germ-free (GF) SW mice were reconstituted with diverse commensal gut microbiota, or monocolonized with gut-specific commensals. Proteomic profiling and quantitative real-time polymerase chain reaction (qRT-PCR) were used to quantify SIgA and IgA levels. 16S rDNA sequencing was carried out to characterize commensal microbiota.

Results

Commensal presence regu

SUBMITTER: Kugadas A 

PROVIDER: S-EPMC5595225 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature