Analysis of individual cells identifies cell-to-cell variability following induction of cellular senescence.

ABSTRACT: Senescent cells play important roles in both physiological and pathological processes, including cancer and aging. In all cases, however, senescent cells comprise only a small fraction of tissues. Senescent phenotypes have been studied largely in relatively homogeneous populations of cultured cells. In vivo, senescent cells are generally identified by a small number of markers, but whether and how these markers vary among individual cells is unknown. We therefore utilized a combination of single-cell isolation and a nanofluidic PCR platform to determine the contributions of individual cells to the overall gene expression profile of senescent human fibroblast populations. Individual senescent cells were surprisingly heterogeneous in their gene expression signatures. This cell-to-cell variab

SUBMITTER: Wiley CD 

PROVIDER: S-EPMC5595671 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature