Normothermic Microwave Irradiation Induces Death of HL-60 Cells through Heat-Independent Apoptosis.

ABSTRACT: Microwaves have been used in various cancer therapies to generate heat and increase tumor cell temperature; however, their use is limited by their side-effects in normal cells and the acquisition of heat resistance. We previously developed a microwave irradiation method that kills cultured cancer cells, including a human promyelomonocytic leukemia (HL-60) cell line, by maintaining a cellular temperature of 37 °C during treatment. In the present study, we investigated the mechanisms underlying HL-60 cell death during this treatment. The microwave-irradiated HL-60 cells appear to undergo caspase-independent apoptosis, whereby DNA fragmentation was induced by mitochondrial dysfunction-related expression of apoptosis-inducing factor (AIF). Caspase-dependent apoptosis was also interrupted by th

SUBMITTER: Asano M 

PROVIDER: S-EPMC5595850 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature