Multiplex quantitative analysis of microRNA expression via exponential isothermal amplification and conformation-sensitive DNA separation.

ABSTRACT: Expression profiling of multiple microRNAs (miRNAs) generally provides valuable information for understanding various biological processes. Thus, it is necessary to develop a sensitive and accurate miRNA assay suitable for multiplexing. Isothermal exponential amplification reaction (EXPAR) has received significant interest as an miRNA analysis method because of high amplification efficiency. However, EXPAR cannot be used for a broader range of applications owing to limitations such as complexity of probe design and lack of proper detection method for multiplex analysis. Here, we developed a sensitive and accurate multiplex miRNA profiling method using modified isothermal EXPAR combined with high-resolution capillary electrophoresis-based single-strand conformation polymorphism (CE-SSCP). T

SUBMITTER: Na J 

PROVIDER: S-EPMC5595994 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature