Characterization of influenza A viruses with polymorphism in PB2 residues 701 and 702.

ABSTRACT: The 701 and 702 positions of influenza PB2 polymerase subunit are previously shown to have roles on host range. Limited polymorphisms at these two residues are identified in natural isolates, thereby limiting the study of their role in the polymerase. In this study, we generated 31 viable viruses by random mutagenesis at this region, indicating that these positions can tolerate a wide range of amino acids. These mutants demonstrated varying polymerase activities and viral replication rates in mammalian and avian cells. Notably, some mutants displayed enhanced polymerase activity, yet their replication kinetics were comparable to the wild-type virus. Surface electrostatic charge predication on the PB2 structural model revealed that the viral polymerase activity in mammalian cells generally

SUBMITTER: Chin AWH 

PROVIDER: S-EPMC5595998 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature