Effect of dopamine on bone morphogenesis protein-2 expression in human retinal pigment epithelium.

ABSTRACT:

Aim

To investigate the effect of dopamine on bone morphogenesis protein-2 (BMP-2) expression in retinal pigment epithelium (RPE) cells in vitro.

Methods

ARPE-19 cells as a human RPE cell line were cultured with dopamine for different times (2, 4, 6, 8, 12, 16 and 24h) or with different concentrations (0.1, 1, 2, 5, 10, 20, and 100 µg/mL) in vitro. BMP-2 mRNA expression level in ARPE-19 cells was analyzed with real-time polymerase chain reaction (PCR) analysis and BMP-2 protein level was measured with Western blot analysis. The active form of BMP-2 in the culture medium was measured with enzyme-linked immunosorbent assay (ELISA).

Results

The expression level of BMP-2 increased significantly cultured with 20 µg/mL dopamine, at different time points (P</

SUBMITTER: Li HH 

PROVIDER: S-EPMC5596220 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature