TNF signaling and macrophages govern fin regeneration in zebrafish larvae.

ABSTRACT: Macrophages are essential for appendage regeneration after amputation in regenerative species. The molecular mechanisms through which macrophages orchestrate blastema formation and regeneration are still unclear. Here, we use the genetically tractable and transparent zebrafish larvae to study the functions of polarized macrophage subsets during caudal fin regeneration. After caudal fin amputation, we show an early and transient accumulation of pro-inflammatory macrophages concomitant with the accumulation of non-inflammatory macrophages which, in contrast to pro-inflammatory macrophages, remain associated to the fin until the end of the regeneration. Chemical and genetic depletion of macrophages suggested that early recruited macrophages that express TNFα are critical for blastema formatio

SUBMITTER: Nguyen-Chi M 

PROVIDER: S-EPMC5596562 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature