Downregulation of microRNA-145 may contribute to liver fibrosis in biliary atresia by targeting ADD3.

ABSTRACT:

Background and objectives

Biliary atresia (BA) is a pediatric liver disease characterized by fibro-obliteration and obstruction of the extrahepatic biliary system, that invariably leads to cirrhosis and even death, if left untreated for extended time. However, its pathology and etiology still remained unknown. In this study, we tested the expression of adducin 3 (ADD3), the gene identified as a susceptibility gene in BA by GWAS, and uncovered its upstream regulatory microRNA in the pathogenesis of BA.

Methods

In this study, 14 infants with BA and 14 infants with choledochal cyst (CC) were enrolled as experimental group and control group, respectively. ADD3 and microRNA-145 (miR-145) expression profiles in liver tissues of BA and CC were determined using qPCR. Luciferase repo

SUBMITTER: Ye Y 

PROVIDER: S-EPMC5597134 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature