A Novel Cellulase Produced by a Newly Isolated Trichoderma virens.

ABSTRACT: Screening and obtaining a novel high activity cellulase and its producing microbe strain is the most important and essential way to improve the utilization of crop straw. In this paper, we devoted our efforts to isolating a novel microbe strain which could produce high activity cellulase. A novel strain Trichoderma virens ZY-01 was isolated from a cropland where straw is rich and decomposed, by using the soil dilution plate method with cellulose and Congo red. The strain has been licensed with a patent numbered ZL 201210295819.6. The cellulase activity in the cultivation broth could reach up to 7.4 IU/mL at a non-optimized fermentation condition with the newly isolated T. virens ZY-01. The cellulase was separated and purified from the T. virens culture broth through (NH₄)₂SO₄ fractional pr

SUBMITTER: Zeng R 

PROVIDER: S-EPMC5597137 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature