Discovery of numerous novel small genes in the intergenic regions of the Escherichia coli O157:H7 Sakai genome.

ABSTRACT: In the past, short protein-coding genes were often disregarded by genome annotation pipelines. Transcriptome sequencing (RNAseq) signals outside of annotated genes have usually been interpreted to indicate either ncRNA or pervasive transcription. Therefore, in addition to the transcriptome, the translatome (RIBOseq) of the enteric pathogen Escherichia coli O157:H7 strain Sakai was determined at two optimal growth conditions and a severe stress condition combining low temperature and high osmotic pressure. All intergenic open reading frames potentially encoding a protein of ≥ 30 amino acids were investigated with regard to coverage by transcription and translation signals and their translatability expressed by the ribosomal coverage value. This led to discovery of 465 unique, putative novel

SUBMITTER: Hucker SM 

PROVIDER: S-EPMC5597208 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature