Programming molecular self-assembly of intrinsically disordered proteins containing sequences of low complexity.

ABSTRACT: Dynamic protein-rich intracellular structures that contain phase-separated intrinsically disordered proteins (IDPs) composed of sequences of low complexity (SLC) have been shown to serve a variety of important cellular functions, which include signalling, compartmentalization and stabilization. However, our understanding of these structures and our ability to synthesize models of them have been limited. We present design rules for IDPs possessing SLCs that phase separate into diverse assemblies within droplet microenvironments. Using theoretical analyses, we interpret the phase behaviour of archetypal IDP sequences and demonstrate the rational design of a vast library of multicomponent protein-rich structures that ranges from uniform nano-, meso- and microscale puncta (distinct protein dro

SUBMITTER: Simon JR 

PROVIDER: S-EPMC5597244 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature