Visualization of PML nuclear import complexes reveals FG-repeat nucleoporins at cargo retrieval sites.

ABSTRACT: Selective nuclear import in eukaryotic cells involves sequential interactions between nuclear import receptors and phenylalanine-glycine (FG)-repeat nucleoporins. Traditionally, binding of cargoes to import receptors is perceived as a nuclear pore complex independent event, while interactions between import complexes and nucleoporins are thought to take place at the nuclear pores. However, studies have shown that nucleoporins are mobile and not static within the nuclear pores, suggesting that they may become engaged in nuclear import before nuclear pore entry. Here we have studied post-mitotic nuclear import of the tumor suppressor protein PML. Since this protein forms nuclear compartments called PML bodies that persist during mitosis, the assembly of putative PML import complexes can be v

SUBMITTER: Lang A 

PROVIDER: S-EPMC5597291 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature