Sequencing on the SOLiD 5500xl System - in-depth characterization of the GC bias.

ABSTRACT: Different types of sequencing biases have been described and subsequently improved for a variety of sequencing systems, mostly focusing on the widely used Illumina systems. Similar studies are missing for the SOLiD 5500xl system, a sequencer which produced many data sets available to researchers today. Describing and understanding the bias is important to accurately interpret and integrate these published data in various ongoing research projects. We report a particularly strong GC bias for this sequencing system when analyzing a defined gDNA mix of 5 microbes with a wide range of different GC contents (20-72%) when comparing to the expected distribution and Illumina MiSeq data from the same DNA pool. Since we observed this bias already under PCR-free conditions, changing the PCR condition

SUBMITTER: Roeh S 

PROVIDER: S-EPMC5597294 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature