Improving heterologous membrane protein production in Escherichia coli by combining transcriptional tuning and codon usage algorithms.

ABSTRACT: High-level, recombinant production of membrane-integrated proteins in Escherichia coli is extremely relevant for many purposes, but has also been proven challenging. Here we study a combination of transcriptional fine-tuning in E. coli LEMO21(DE3) with different codon usage algorithms for heterologous production of membrane proteins. The overexpression of 6 different membrane proteins is compared for the wild-type gene codon usage variant, a commercially codon-optimized variant, and a codon-harmonized variant. We show that transcriptional fine-tuning plays a major role in improving the production of all tested proteins. Moreover, different codon usage variants significantly improved production of some of the tested proteins. However, not a single algorithm performed consistently best for t

SUBMITTER: Claassens NJ 

PROVIDER: S-EPMC5597330 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature