Investigation of the effects of starch on the physical and biological properties of polyacrylamide (PAAm)/starch nanofibers.

ABSTRACT: Here, we report the development of a new polyacrylamide (PAAm)/starch nanofibers' blend system and highlight its potential as substrate for efficient enzyme immobilization. PAAm was synthesized and blended with starch. The final blend was then electrospun into nanofibers. The response surface methodology was used to analyze the parameters that control nanofiber's diameter. Electrospun mat was then modified either by cross-linking or phytase immobilization using silane coupling agent and glutaraldehyde chemistry. Physico-chemical properties of blends were investigated using spectroscopic and thermal studies. The evaluation of immobilized enzyme kinetics on both pure and the starch blended PAAm nanofibers was performed using Michaelis-Menten kinetic curves. Fourier transform infrared spectro

SUBMITTER: Taghavian H 

PROVIDER: S-EPMC5597571 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature