The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3.

ABSTRACT: The centromere is the chromosomal locus at which the kinetochore is assembled to direct chromosome segregation. The histone H3 variant, centromere protein A (CENP-A), is known to epigenetically mark active centromeres, but the mechanism by which CENP-A propagates at the centromere, replacing histone H3, remains poorly understood. Using fission yeast, here we show that the Ino80 adenosine triphosphate (ATP)-dependent chromatin-remodeling complex, which removes histone H3-containing nucleosomes from associated chromatin, promotes CENP-A^Cnp1 chromatin assembly at the centromere in a redundant manner with another chromatin-remodeling factor Chd1^Hrp1. CENP-A^Cnp1 chromatin actively recruits the Ino80 complex to centromeres to elicit eviction of histone H3-contain

SUBMITTER: Choi ES 

PROVIDER: S-EPMC5597579 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature