Consistent View of Polypeptide Chain Expansion in Chemical Denaturants from Multiple Experimental Methods.

ABSTRACT: There has been a long-standing controversy regarding the effect of chemical denaturants on the dimensions of unfolded and intrinsically disordered proteins: A wide range of experimental techniques suggest that polypeptide chains expand with increasing denaturant concentration, but several studies using small-angle X-ray scattering (SAXS) have reported no such increase of the radius of gyration (Rg). This inconsistency challenges our current understanding of the mechanism of chemical denaturants, which are widely employed to investigate protein folding and stability. Here, we use a combination of single-molecule Förster resonance energy transfer (FRET), SAXS, dynamic light scattering (DLS), and two-focus fluorescence correlation spectroscopy (2f-FCS) to characterize the denaturant dependenc

SUBMITTER: Borgia A 

PROVIDER: S-EPMC5597961 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature