A Multicenter Study To Evaluate the Performance of High-Throughput Sequencing for Virus Detection.

ABSTRACT: The capability of high-throughput sequencing (HTS) for detection of known and unknown viruses makes it a powerful tool for broad microbial investigations, such as evaluation of novel cell substrates that may be used for the development of new biological products. However, like any new assay, regulatory applications of HTS need method standardization. Therefore, our three laboratories initiated a study to evaluate performance of HTS for potential detection of viral adventitious agents by spiking model viruses in different cellular matrices to mimic putative materials for manufacturing of biologics. Four model viruses were selected based upon different physical and biochemical properties and commercial availability: human respiratory syncytial virus (RSV), Epstein-Barr virus (EBV), feline le

SUBMITTER: Khan AS 

PROVIDER: S-EPMC5597969 | biostudies-literature | 2017 Sep-Oct

REPOSITORIES: biostudies-literature