A comparison of sequencing platforms and bioinformatics pipelines for compositional analysis of the gut microbiome.

ABSTRACT:

Background

Advancements in Next Generation Sequencing (NGS) technologies regarding throughput, read length and accuracy had a major impact on microbiome research by significantly improving 16S rRNA amplicon sequencing. As rapid improvements in sequencing platforms and new data analysis pipelines are introduced, it is essential to evaluate their capabilities in specific applications. The aim of this study was to assess whether the same project-specific biological conclusions regarding microbiome composition could be reached using different sequencing platforms and bioinformatics pipelines.

Results

Chicken cecum microbiome was analyzed by 16S rRNA amplicon sequencing using Illumina MiSeq, Ion Torrent PGM, and Roche 454 GS FLX Titanium platforms, with standard and modified prot

SUBMITTER: Allali I 

PROVIDER: S-EPMC5598039 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature