One-step generation of cell-laden microgels using double emulsion drops with a sacrificial ultra-thin oil shell.

ABSTRACT: Cell-laden microgels with highly uniform sizes have significant potential in tissue engineering and cell therapy due to their capability to provide a physiologically relevant three-dimensional (3D) microenvironment for living cells. In this work, we present a simple and efficient microfluidic approach to produce monodisperse cell-laden microgels through the use of double emulsion drops with an ultra-thin oil shell as the sacrificial template. Specifically, the thin oil shell in double emulsion spontaneously dewets upon polymerization of the innermost precursor drop and subsequent transfer into an aqueous solution, resulting in direct dispersion of microgels in the aqueous phase. Compared to conventional single emulsion-based techniques for cell encapsulation, this one-step approach prevent

SUBMITTER: Choi CH 

PROVIDER: S-EPMC5598084 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature