Effect of Nɛ-carboxymethyllysine on oxidative stress and the glutathione system in beta cells.

ABSTRACT: One of the pathways involved in the pathogenesis of diabetic complications is the formation of excessive levels of advanced glycation end (AGE) products. N^ɛ-carboxymethyllysine (CML) is one of the best-characterized AGEs. Because little is known about the effects of AGEs on pancreatic beta cells, we investigated the effect of CML on human pancreatic cells and determined the activity and gene expression of glutathione system components. CML at a concentration of 0.5 mM induced cell death in human pancreatic beta cells, which was accompanied by increased intracellular oxidative stress. No changes in the gene expression of the receptor for AGEs (RAGE) were found, although an increase in the level of a target cytokine of RAGE after CML exposure was observed. Additionally we found th

SUBMITTER: Boesten DMPHJ 

PROVIDER: S-EPMC5598217 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature