Proapoptotic activity of aflatoxin B₁ and sterigmatocystin in HepG2 cells.

ABSTRACT: Aflatoxin B₁ (AFB₁) and sterigmatocystin (ST) are two hepatocarcinogenic mycotoxins that are commonly coexisted in cereal grains, and their co-proapoptotic activity in HepG2 cells was studied. The values of IC₅₀, which is the dosage of mycotoxin resulting in a 50% cell growth inhibition measured by a sulforhodamine B (SRB) colorimetric assay, were 16.9 μM and 7.3 μM for AFB₁ and ST, respectively. Additively and dose-dependently, cell apoptosis-related toxicity endpoints of double strand DNA and ATP content were decreased while the intracellular ROS and mitochondria membrane permeability (MMP) were increased. Consistently, when cell cycle is arrest at G0/G1 or S phase by AFB₁ and/or ST, the experimental results from flow cytometry assay

SUBMITTER: Liu Y 

PROVIDER: S-EPMC5598229 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature