Purinergic signaling mediates oxidative stress in UVA-exposed THP-1 cells.

ABSTRACT: Ultraviolet A (UVA) radiation, the major UV component of solar radiation, can penetrate easily to the dermis, where it causes significant damage to cellular components by inducing formation of reactive oxygen species (ROS). On the other hand, extracellular ATP is released in response to various stimuli, and activates purinergic P2X7 receptor, triggering ROS production and cell death. Here, we examined the hypothesis that ATP release followed by activation of P2X7 receptor plays a role in UVA-induced oxidative cell damage, using human acute monocytic leukemia cell line THP-1. Indeed, UVA irradiation of THP-1 cells induced ATP release and activation of P2X7 receptor. Irradiated cells showed a rapid increase of both p67 ^phox in membrane fraction and intracellular ROS. Pretre

SUBMITTER: Kawano A 

PROVIDER: S-EPMC5598242 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature