In vitro pepsin resistance of proteins: Effect of non-reduced SDS-PAGE analysis on fragment observation.

ABSTRACT: The introduction of novel proteins to food products carries with it the need to assess the potential allergenicity of such materials. Resistance to in vitro pepsin digestion is one parameter considered in such a risk assessment using a weight of evidence approach; however, the methodology used to investigate this has not been fully standardised. In vitro pepsin resistance assays typically involve SDS-PAGE performed under reducing conditions, with limited published data available on the assay using non-reducing conditions despite the need to consider non-reducing analysis techniques having been highlighted by regulatory bodies such as the European Food Safety Authority (EFSA). The purpose of the work reported here was to investigate the applicability of (and additional insight

SUBMITTER: Pickles J 

PROVIDER: S-EPMC5598364 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature