Short-term changes in intracellular ROS localisation after the silver nanoparticles exposure depending on particle size.

ABSTRACT: Silver nanoparticles (AgNPs) induce the production of reactive oxygen species (ROS) and apoptosis. These effects are enhanced by smaller particles. Using live-cell imaging, we show that AgNPs induced ROS production rapidly in a size-dependent manner after exposure of cells to 70-nm and 1-nm AgNPs (AgNPs-70, AgNPs-1), but not AgNO₃. Exposure of cells to 5 μg/mL each of AgNPs-70, AgNPs-1 or AgNO₃ for 1 h decreased the cell viability by approximately 40%, 100% and 20%, respectively. ROS were rapidly induced after 5 and 60 min by AgNPs-1 and AgNPs-70, respectively, whereas AgNO₃ had no detectable effect. ROS production detected using the reporter dichlorodihydrofluorescein was observed in whole cells and mitochondria 5 and 60 min after exposure to AgNPs-1. The

SUBMITTER: Onodera A 

PROVIDER: S-EPMC5598391 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature