Technical variability of 2D gel electrophoresis - Application to soybean allergens.

ABSTRACT: Two-dimensional gel electrophoresis (2-DE) technique is used as a performing technique to assess the variability of protein expression in crops, and especially soybean endogenous food allergens, which are a subset of proteins of interest for assessing whether genetically modified (GM) soybean has a different allergenic profile compared to its non-GM counterpart. On top of the biological variability of the 2-DE, which has already been studied by several laboratories, technical variability has to be evaluated. In this study, several sources of variability (number of gel replicates, protein extracts, study timings and operators) were assessed qualitatively and quantitatively on all detectable polypeptide spots as well as on food allergen spots. Results showed that the major source of variabil

SUBMITTER: Pallen C 

PROVIDER: S-EPMC5598412 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature