1:1 FASTA update: Using the power of E-values in FASTA to detect potential allergen cross-reactivity.

ABSTRACT: In the context of regulatory assessment of transgenic proteins for potential allergenicity, a previous investigation demonstrated that a 1:1 FASTA comparison using an E-value of 1.0E-09 as a criterion is superior to the conventional FASTA search (using the whole sequence as a query) for >35% identity over 80 amino acids, but with improved specificity. A further study, using groups of known cross-reactive peanut allergens, indicates the sensitivity of this approach is superior to the conventional FASTA search and equivalent to 80-mer sliding window FASTA search recommended by WHO/FAO. Specifically, the 1:1 FASTA approach eliminated the technical issues resulting from lack of identification of short query sequences with high identity to known allergens, or high identity over short ami

SUBMITTER: Song P 

PROVIDER: S-EPMC5598423 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature