Cell-Free DNA and DNase Activity in Dogs with Immune-Mediated Hemolytic Anemia.

ABSTRACT:

Background

Immune-mediated hemolytic anemia (IMHA) in dogs has a high risk of thrombosis and is associated with marked neutrophilia and necrosis. Cell death and release of neutrophil extracellular traps contribute to increased serum concentrations of cell-free DNA, and in human autoimmune disease reduced DNase activity further increases cell-free DNA. Free DNA in blood has prothrombotic properties and could contribute to hypercoagulability in IMHA.

Hypothesis

Cell-free DNA is elevated and DNase activity reduced in dogs with IMHA compared to healthy dogs.

Animals

Dogs presenting to two referral hospitals with IMHA (n = 28) and healthy controls (n = 20).

Methods

Prospective observational study. Blood was collected and death and thrombotic events occurring in the

SUBMITTER: Jeffery U 

PROVIDER: S-EPMC5598899 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature