Expression of surfactant protein B is dependent on cell density in H441 lung epithelial cells.

ABSTRACT:

Background

Expression of surfactant protein (SP)-B, which assures the structural stability of the pulmonary surfactant film, is influenced by various stimuli, including glucocorticoids; however, the role that cell-cell contact plays in SP-B transcription remains unknown. The aim of the current study was to investigate the impact of cell-cell contact on SP-B mRNA and mature SP-B expression in the lung epithelial cell line H441.

Methods

Different quantities of H441 cells per growth area were either left untreated or incubated with dexamethasone. The expression of SP-B, SP-B transcription factors, and tight junction proteins were determined by qPCR and immunoblotting. The influence of cell density on SP-B mRNA stability was investigated using the transcription inhibitor actinom

SUBMITTER: Fehrholz M 

PROVIDER: S-EPMC5599067 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature