Foam separation of Rhodamine-G and Evans Blue using a simple separatory bottle system.

ABSTRACT: A simple separatory glass bottle was used to improve separation effectiveness and cost efficiency while simultaneously creating a simpler system for separating biological compounds. Additionally, it was important to develop a scalable separation method so this would be applicable to both analytical and preparative separations. Compared to conventional foam separation methods, this method easily forms stable dry foam which ensures high purity of yielded fractions. A negatively charged surfactant, sodium dodecyl sulfate (SDS), was used as the ligand to carry a positively charged Rhodamine-G, leaving a negatively charged Evans Blue in the bottle. The performance of the separatory bottle was tested for separating Rhodamine-G from Evans Blue with sample sizes ranged from 1 to 12mg in preparativ

SUBMITTER: Dasarathy D 

PROVIDER: S-EPMC5599151 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature