The importance of simulated lung fluid (SLF) extractions for a more relevant evaluation of the oxidative potential of particulate matter.

ABSTRACT: Particulate matter (PM) induces oxidative stress in vivo, leading to adverse health effects. Oxidative potential (OP) of PM is increasingly studied as a relevant metric for health impact (instead of PM mass concentration) as much of the ambient particle mass do not contribute to PM toxicity. Several assays have been developed to quantify PM oxidative potential and a widely used one is the acellular dithiothreitol (DTT) assay. However in such assays, particles are usually extracted with methanol or Milli-Q water which is unrepresentative of physiological conditions. For this purpose, OP_DTT measurements after simulated lung fluids (SLF) extraction, in order to look at the impact of simulated lung fluid constituents, were compared to Milli-Q water extraction measurements. Our major

SUBMITTER: Calas A 

PROVIDER: S-EPMC5599505 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature