A NMDA-receptor calcium influx assay sensitive to stimulation by glutamate and glycine/D-serine.

ABSTRACT: N-methyl-D-aspartate-receptors (NMDARs) are ionotropic glutamate receptors that function in synaptic transmission, plasticity and cognition. Malfunction of NMDARs has been implicated in a variety of nervous system disorders, making them attractive therapeutic targets. Overexpression of functional NMDAR in non-neuronal cells results in cell death by excitotoxicity, hindering the development of cell-based assays for NMDAR drug discovery. Here we report a plate-based, high-throughput approach to study NMDAR function. Our assay enables the functional study of NMDARs with different subunit composition after activation by glycine/D-serine or glutamate and hence presents the first plate-based, high throughput assay that allows for the measurement of NMDAR function in glycine/D-serine and/or gluta

SUBMITTER: Guo H 

PROVIDER: S-EPMC5599507 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature