A CRISPR screen identifies genes controlling Etv2 threshold expression in murine hemangiogenic fate commitment.

ABSTRACT: The ETS transcription factor Etv2 is necessary and sufficient for the generation of hematopoietic and endothelial cells. However, upstream regulators of Etv2 in hemangiogenesis, generation of hematopoietic and endothelial cells, have not been clearly addressed. Here we track the developmental route of hemangiogenic progenitors from mouse embryonic stem cells, perform genome-wide CRISPR screening, and transcriptome analysis of en route cell populations by utilizing Brachyury, Etv2, or Scl reporter embryonic stem cell lines to further understand the mechanisms that control hemangiogenesis. We identify the forkhead transcription factor Foxh1, in part through Eomes, to be critical for the formation of FLK1⁺ mesoderm, from which the hemangiogenic fate is specified. Importantly, heman

SUBMITTER: Zhao H 

PROVIDER: S-EPMC5599515 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature