STIM1-dependent Ca²⁺ signaling regulates podosome formation to facilitate cancer cell invasion.

ABSTRACT: The clinical significance of STIM proteins and Orai Ca²⁺ channels in tumor progression has been demonstrated in different types of cancers. Podosomes are dynamic actin-rich cellular protrusions that facilitate cancer cell invasiveness by degrading extracellular matrix. Whether STIM1-dependent Ca²⁺ signaling facilitates cancer cell invasion through affecting podosome formation remains unclear. Here we show that the invasive fronts of cancer tissues overexpress STIM1, accompanied by active store-operated Ca²⁺ entry (SOCE). Interfering SOCE activity by SOCE inhibitors and STIM1 or Orai1 knockdown remarkably affects podosome rosettes formation. Mechanistically, STIM1-silencing significantly alters the podosome rosettes dynamics, shortens the maintenance phase o

SUBMITTER: Chen YW 

PROVIDER: S-EPMC5599537 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature