Transcriptome analysis of developing lens reveals abundance of novel transcripts and extensive splicing alterations.

ABSTRACT: Lens development involves a complex and highly orchestrated regulatory program. Here, we investigate the transcriptomic alterations and splicing events during mouse lens formation using RNA-seq data from multiple developmental stages, and construct a molecular portrait of known and novel transcripts. We show that the extent of novelty of expressed transcripts decreases significantly in post-natal lens compared to embryonic stages. Characterization of novel transcripts into partially novel transcripts (PNTs) and completely novel transcripts (CNTs) (novelty score ≥ 70%) revealed that the PNTs are both highly conserved across vertebrates and highly expressed across multiple stages. Functional analysis of PNTs revealed their widespread role in lens developmental processes while hundreds of CNT

SUBMITTER: Srivastava R 

PROVIDER: S-EPMC5599659 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature