Panels of HIV-1 Subtype C Env Reference Strains for Standardized Neutralization Assessments.

ABSTRACT: In the search for effective immunologic interventions to prevent and treat HIV-1 infection, standardized reference reagents are a cost-effective way to maintain robustness and reproducibility among immunological assays. To support planned and ongoing studies where clade C predominates, here we describe three virus panels, chosen from 200 well-characterized clade C envelope (Env)-pseudotyped viruses from early infection. All 200 Envs were expressed as a single round of replication pseudoviruses and were tested to quantify neutralization titers by 16 broadly neutralizing antibodies (bnAbs) and sera from 30 subjects with chronic clade C infections. We selected large panels of 50 and 100 Envs either to characterize cross-reactive breadth for sera identified as having potent neutralization acti

SUBMITTER: Hraber P 

PROVIDER: S-EPMC5599761 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature