The impact of von Willebrand factor on factor VIII memory immune responses.

ABSTRACT: Immune tolerance induction (ITI) with aggressive infusion of factor VIII (FVIII) is the current strategy used to eradicate FVIII inhibitors and restore normal FVIII pharmacokinetics in inhibitor patients. Whether the use of FVIII products containing von Willebrand factor (VWF) will affect the efficacy of ITI is still controversial. In this study, we explored the impact of VWF on FVIII memory immune responses in hemophilia A (HA) mice. A T-cell proliferation assay and cytokine profile analysis were used to study FVIII-primed CD4⁺ T cells. When CD4⁺ T cells from primed FVIII^null mice were restimulated with recombinant human FVIII (rhF8) plus recombinant human VWF (rhVWF) in vitro, the percentages of daughter CD4⁺ T cells were significantly decrease

SUBMITTER: Chen J 

PROVIDER: S-EPMC5600162 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature